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Påvisning af en cirkulerende mikroRNA brugerdefineret Panel hos patienter med metastatisk kolorektal Cancer
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Påvisning af en cirkulerende mikroRNA brugerdefineret Panel hos patienter med metastatisk kolorektal Cancer

Article DOI: 10.3791/58615-v • 08:12 min • March 14th, 2019
March 14th, 2019 •
1, 1, 2, 3, 1
1Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, 2Department of Medical Oncology, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, 3Unit of Biostatistics and Clinical Trials, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS

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Vi præsenterer en protokol for at evaluere udtryk niveauer af cirkulerende mikroRNA (miRNAs) i plasmaprøver fra patienter. Navnlig, brugte vi et kommercielt tilgængeligt kit for cirkulerende miRNA udvinding og omvendt transkription. Endelig, vi analyseret et panel af 24 udvalgte miRNAs ved hjælp af real-time pre plettede sonde brugerdefinerede plader.

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Kræftforskning sag 145 plasma MicroRNA metastatisk kolorektal cancer bevacizumab RT-PCR avanceret kemi brugerdefineret panel
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