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4D-mikroskopi af gær
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4D-mikroskopi af gær

Article DOI: 10.3791/58618-v • 12:00 min • April 28th, 2019
April 28th, 2019 •
1, 1
1Department of Molecular Genetics and Cell Biology, University of Chicago

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Denne protokol beskriver analysen af fluorescently mærkede intracellulære rum i spirende gær ved hjælp af multi-Color 4D (time-lapse 3D) confokal mikroskopi. Billedbehandlings parametrene vælges til at opfange passende signaler, samtidig med at foto skader begrænses. Brugerdefinerede ImageJ plugins tillader, at mærkede strukturer spores og analyseres kvantitativt.

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Biologi Confokal mikroskopi 4D-billeddannelse fluorescens foto blegning dekoncentrering ImageJ gær
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