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4D microscopia de levedura
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4D microscopia de levedura

Article DOI: 10.3791/58618-v • 12:00 min • April 28th, 2019
April 28th, 2019 •
1, 1
1Department of Molecular Genetics and Cell Biology, University of Chicago

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Este protocolo descreve a análise de compartimentos intracelular cDNAs etiquetados no fermento de brotamento usando a microscopia confocal 4D (Time-Lapse 3D) multi-color. Os parâmetros de imagem são escolhidos para capturar sinais adequados, limitando o Fotodano. Os plugins personalizados do ImageJ permitem que as estruturas rotuladas sejam rastreadas e analisadas quantitativamente.

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Biologia edição 146 microscopia confocal imagem 4D fluorescência fotobranqueamento desvolução ImageJ levedura
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