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Journal / Developmental Biology / Fluorimetri teknikker til vurdering af Sperm…
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Developmental Biology

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JoVE Journal Developmental Biology
Fluorimetri teknikker til vurdering af Sperm membraner
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Fluorimetri teknikker til vurdering af Sperm membraner

Article DOI: 10.3791/58622-v • 08:58 min • November 28th, 2018
November 28th, 2018 •
1,2, 1,2
1Animal Sperm Research Center, Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, 2Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem

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Vi præsenterer her, metoder til at vurdere spermatozoan membran integritet, en cellulær funktion tilknyttet sperm befrugtning kompetence. Vi beskriver tre teknikker for fluorimetri vurdering af sperm membraner: samtidige farvning med specifikke fluorescerende sonder, Fluorescens mikroskopi og avancerede sæd-dedikeret flowcytometri. Eksempler på kombinerer metoder er også præsenteret.

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Udviklingsmæssige biologi sag 141 Sperm acrosome reaktion mitokondrielle membran potentiale plasma membran fluorescerende sonde flowcytometri sæd evaluering
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