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Journal / Developmental Biology / Fluorimetric teknikker for vurdering av Sperm…
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JoVE Journal Developmental Biology
Fluorimetric teknikker for vurdering av Sperm membraner
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Fluorimetric teknikker for vurdering av Sperm membraner

Article DOI: 10.3791/58622-v • 08:58 min • November 28th, 2018
November 28th, 2018 •
1,2, 1,2
1Animal Sperm Research Center, Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, 2Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem

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Her presenterer vi metoder for å evaluere spermatozoan membran integritet, en cellular funksjon knyttet sperm befruktning kompetanse. Vi beskriver tre teknikker for fluorimetric vurdering av sæd membraner: samtidige flekker med bestemte fluorescerende sonder, fluorescens mikroskopi og avanserte sæd-dedikert flowcytometri. Eksempler på kombinerte metodikkene er også presentert.

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Utviklingspsykologi biologi problemet 141 Sperm acrosome reaksjon mitokondrie membran potensialet plasma membran fluorescerende sonde flowcytometri sæd evaluering
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