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Fluorimetric teknikleri Sperm membran değerlendirilmesi için
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Fluorimetric teknikleri Sperm membran değerlendirilmesi için

Article DOI: 10.3791/58622-v • 08:58 min • November 28th, 2018
November 28th, 2018 •
1,2, 1,2
1Animal Sperm Research Center, Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, 2Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem

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Burada, metodolojileri spermatozoan membran bütünlüğü, sperm fertilizasyon yetki ile ilişkili bir hücresel özellik değerlendirmek için mevcut. Biz sperm membran fluorimetric değerlendirilmesi için üç teknikler tarif: eşzamanlı belirli floresan problar, floresans mikroskobu ve gelişmiş sperm adanmış akış sitometresi ile boyama. Örnek yöntemleri birleştirerek da sunulmaktadır.

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Gelişim biyolojisi sayı: 141 Sperm acrosome tepki mitokondri zar potansiyeli plazma zarı floresan sonda akış sitometresi meni değerlendirme
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