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Journal / Genetics / Cell Based Assays of SINEUP Non-coding RNAs That…
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Genetics

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Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

Article DOI: 10.3791/58627-v • 10:21 min • February 1st, 2019
February 1st, 2019 •
*1, *1, 1,2
1Laboratory for Transcriptome Technology, RIKEN Center for Integrative Medical Sciences, 2TransSINE Technologies Co. Ltd.
* These authors contributed equally

Chapters

Summary
Automatic Translation

SINEUPs are synthetic antisense non-coding RNAs, which contain a binding domain (BD) and an effector domain (ED) and up-regulate translation of target mRNA. Here, we describe detection methods for SINEUPs in cultured cell lines, analysis of their translation-promoting activity by Western-blot and a semi-automated high throughput imaging system.

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Cell Based Assays SINEUP Non-coding RNAs MRNA Translation Gene Activity Moderation Deficiencies Compensation High Translation From MRNAs Global Screening Of Target Genes Living Cells Imaging Therapy For Haploinsufficient Instances Frataxin Deficiency In Friedrich's Ataxia Zenbu Browser Fantom Project Database Transcription Start Site (TSS) Expression Levels In Cells And Tissues PARK7 MRNA Analysis Cage Peak Region Cell And Tissue Specific Transcription Expression Levels Binding Domain Sequences
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