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Journal / Biochemistry / Isolamento dei Tessuti Vescicoli Extracellulari…
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Biochemistry

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JoVE Journal Biochemistry
Isolamento dei Tessuti Vescicoli Extracellulari dal Fegato
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Isolamento dei Tessuti Vescicoli Extracellulari dal Fegato

Article DOI: 10.3791/58649-v • 05:39 min • August 21st, 2019
August 21st, 2019 •
1, 1, 1
1Department of Transplantation, Mayo Clinic

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Questo è un protocollo per isolare le vesciche extracellulari del tessuto (EV) dal fegato. Il protocollo descrive un processo in due fasi che coinvolge la perfusione di collagenasi seguito da ultracentrifugazione differenziale per isolare gli EV del tessuto epatico.

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Biochimica Numero 150 Fegato vescicoli extracellulari collagenase ultra-centrifugazione diagnostica perfusione
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