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Journal / Environment / Reproduserbar dsRNA Microinjection og Oviposition …
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Environment

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JoVE Journal Environment
Reproduserbar dsRNA Microinjection og Oviposition Bioassay i mygg og huse fluene
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Reproduserbar dsRNA Microinjection og Oviposition Bioassay i mygg og huse fluene

Article DOI: 10.3791/58650-v • 08:41 min • November 8th, 2018
November 8th, 2018 •
1, 2,3, 1, 1,2, 1
1USDA/ARS Center for Medical, Agricultural, and Veterinary Entomology, 2CMAVE Detachment, Navy Entomology Center of Excellence, 3Lovelace Respiratory Research Institute

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Denne protokollen beskriver en microinjection metode som vi har standardisert og brukt i flere år for å levere bestemte mengder nukleinsyrer direkte til hemolymph av mygg og huse fluene. Denne protokollen gir minimal injeksjon dødelighet og lar dose korrelert målinger av fruktbarhet.

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