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Nerve Stem Cell Differentiation by a One-step Cold Atmospheric Plasma Treatment In Vitro
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Nerve Stem Cell Differentiation by a One-step Cold Atmospheric Plasma Treatment In Vitro

Article DOI: 10.3791/58663-v • 07:01 min • January 11th, 2019
January 11th, 2019 •
*1, *2, 3
1State Key Laboratory of Advanced Electromagnetic Engineering and Technology, Huazhong University of Science and Technology, 2College of Life Science and Health, Wuhan University of Science and Technology, 3Department of Pathophysiology, Beijing Neurosurgical Institute/Beijing Tiantan Hospital, Capital Medical University
* These authors contributed equally

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Summary
Automatic Translation

This protocol aims to provide detailed experimental steps of a cold atmospheric plasma treatment on neural stem cells and immunofluorescence detection for differentiation enhancement.

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Nerve Stem Cells Differentiation Cold Atmospheric Plasma Treatment In Vitro Neurological Disease Parkinson's Disease Directed Differentiation Tissue Transplantation Cell Damage Murine Neural Stem Cells DMEM Confluency PBS Trypsin Culture Medium Single Cell Suspension Poly-D-lysine Coated Cover Slip Cell Density Microscope Incubator
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