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Journal / Biochemistry / Hvordan til at stabilisere Protein: stabilitet…
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Biochemistry

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JoVE Journal Biochemistry
Hvordan til at stabilisere Protein
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Hvordan til at stabilisere Protein: stabilitet skærme til termiske Skift Assays og Nano Differential Scanning Fluorimetry i projektets Virus-X

Article DOI: 10.3791/58666-v • 07:22 min • February 11th, 2019
February 11th, 2019 •
1, 1, 2, 1,2
1Department of Biosciences, Durham University, 2Department of Chemistry, Durham University

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En protokol præsenteres du kan hurtigt teste den termiske stabilitet af proteiner i en række betingelser gennem termisk Skift assays og nano differential scanning fluorimetry. Buffersystemer, salte og tilsætningsstoffer, sammen er bestående af tre enestående stabilitet skærme, analyseret med proteiner til at identificere egnede buffere for funktionelle og strukturelle studier.

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Biokemi spørgsmålet 144 DSF nanoDSF TSA protein stabilitet protein oprensning Proteinkrystallografi
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