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Journal / Biochemistry / Verwendung von hohen Gehalt Imaging zu Target…
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Biochemistry

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JoVE Journal Biochemistry
Verwendung von hohen Gehalt Imaging zu Target Engagement in adhärenten Zellen zu quantifizieren
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Verwendung von hohen Gehalt Imaging zu Target Engagement in adhärenten Zellen zu quantifizieren

Article DOI: 10.3791/58670-v • 07:23 min • November 29th, 2018
November 29th, 2018 •
1,2, 1,2, 1,3
1Chemical Biology Consortium Sweden, Science for Life Laboratory, Karolinska Institutet, Solna, 2Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Solna, 3Science for Life Laboratory, Department of Oncology-Pathology, Karolinska Institutet, Stockholm

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Messungen der Droge Ziel Engagement sind von zentraler Bedeutung für effektive Medikamentenentwicklung und chemische Sonde Validierung. Hier zeigen wir ein Protokoll zur Messung der Droge-Ziel Engagement mit hohen Inhalt Bildgebung in einer Mikrotestplatte-kompatiblen Adaption des zellulären thermische Verschiebung Assays (CETSA).

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Biochemie Ausgabe 141 zelluläre thermische Verschiebung Assay (CETSA) Imaging Ziel Engagement p38α High Throughput Screening Einzelzelle Auflösung
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