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Journal / Genetics / Chromatin Immunoprecipitation av Murine brun…
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JoVE Journal Genetics
Chromatin Immunoprecipitation av Murine brun fettvev
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Chromatin Immunoprecipitation av Murine brun fettvev

Article DOI: 10.3791/58682-v • 07:50 min • November 21st, 2018
November 21st, 2018 •
1, 1, 2, 1
1Biochemistry Department, Boston University School of Medicine, 2Bioinformatic Hub, Boston University

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Her beskriver vi en protokoll for effektiv chromatin immunoprecipitation (ChIP) etterfulgt av høy gjennomstrømming DNA sekvensering (ChIP-seq) av brun fettvev (BAT) isolert fra mus. Denne protokollen er egnet for både kartlegging histone modifikasjoner og undersøker genomet hele lokalisering av ikke-histone proteiner av interesse i vivo.

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Genetikk problemet 141 Chromatin immunoprecipitation brun fettvev neste generasjons sekvensering mus fett
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