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Journal / Genetics / Immunoprecipitazione della cromatina del tessuto…
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JoVE Journal Genetics
Immunoprecipitazione della cromatina del tessuto adiposo marrone murino
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Immunoprecipitazione della cromatina del tessuto adiposo marrone murino

Article DOI: 10.3791/58682-v • 07:50 min • November 21st, 2018
November 21st, 2018 •
1, 1, 2, 1
1Biochemistry Department, Boston University School of Medicine, 2Bioinformatic Hub, Boston University

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Qui descriviamo un protocollo per immunoprecipitazione efficiente della cromatina (ChIP) seguito da sequenziamento del DNA di alto-rendimento (ChIP-seq) del tessuto adiposo marrone (pipistrello) isolato da un mouse. Questo protocollo è adatto sia per mappatura modificazioni istoniche e indagando genoma localizzazione delle proteine non istoniche di interesse in vivo.

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Tessuto adiposo di genetica problema 141 cromatina immunoprecipitazione marrone sequenziamento mouse grasso di nuova generazione
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