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Visualization of 3D White Adipose Tissue Structure Using Whole-mount Staining

Article DOI: 10.3791/58683-v • 06:20 min • November 17th, 2018
November 17th, 2018 •
*1, *1, 1,2, 1, 3, 4, 1,2,5
1Translational Medicine Program, The Hospital for Sick Children, 2Department of Laboratory Medicine and Pathobiology, University of Toronto, 3Neurosciences & Mental Health Program, The Hospital for Sick Children, 4Department of Biochemistry and Molecular Biology, Smart-Aging Convergence Research Center, College of Medicine, Yeungnam University, 5Banting and Best Diabetes Centre, University of Toronto
* These authors contributed equally

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Summary
Automatic Translation

The focus of the present study is to demonstrate the whole-mount immunostaining and visualization technique as an ideal method for 3D imaging of adipose tissue architecture and cellular component.

Tags

Visualization 3D White Adipose Tissue Whole-mount Staining Intercellular Interactions Adipose Tissue Physiology Metabolic Conditions Fasting Obesity Technique Adipose 3D Structure Immunohistochemistry Organ Systems Nervous System Brain Fixation Period Antibody Concentrations Dissection Paraformaldehyde Cell Culture Plate Washing PBS-0.3T
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