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Journal / Developmental Biology / RNA gebaseerde herprogrammering van menselijke…
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Developmental Biology

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JoVE Journal Developmental Biology
RNA gebaseerde herprogrammering van menselijke primaire fibroblasten in geïnduceerde pluripotente stamcellen
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RNA gebaseerde herprogrammering van menselijke primaire fibroblasten in geïnduceerde pluripotente stamcellen

Article DOI: 10.3791/58687-v • 11:39 min • November 26th, 2018
November 26th, 2018 •
1,2,3, 1,2, 1,2,3, 1,2,3
1Department of Dermatology, University of Colorado School of Medicine, Anschutz Medical Campus, 2Charles C. Gates Center for Regenerative Medicine, University of Colorado School of Medicine, Anschutz Medical Campus, 3Stem Cell Biobank and Disease Modeling Core, University of Colorado School of Medicine, Anschutz Medical Campus

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Hier beschrijven we een klinisch relevante, hoog rendement, feeder-vrije methode om te herprogrammeren menselijke primaire fibroblasten in geïnduceerde pluripotente stamcellen met behulp van gemodificeerde mRNAs codering herprogrammering factoren en volwassen microRNA-367/302 nabootst. Ook inbegrepen zijn methoden om herprogrammering efficiëntie, te beoordelen vouw klonale iPSC kolonies en bevestigen van de expressie van de pluripotent markering TRA-1-60.

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Ontwikkelingsbiologie kwestie 141 ingegeven pluripotente cellen van de stam (iPSCs) herprogrammering menselijke bewerkt mRNAs klinisch relevante microRNAs (miRNAs) fibroblasten
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