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Developmental Biology

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JoVE Journal Developmental Biology
Basado en el ARN la reprogramación de fibroblastos primarios de humanos en células madre pluripotentes inducidas
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Basado en el ARN la reprogramación de fibroblastos primarios de humanos en células madre pluripotentes inducidas

Article DOI: 10.3791/58687-v • 11:39 min • November 26th, 2018
November 26th, 2018 •
1,2,3, 1,2, 1,2,3, 1,2,3
1Department of Dermatology, University of Colorado School of Medicine, Anschutz Medical Campus, 2Charles C. Gates Center for Regenerative Medicine, University of Colorado School of Medicine, Anschutz Medical Campus, 3Stem Cell Biobank and Disease Modeling Core, University of Colorado School of Medicine, Anschutz Medical Campus

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Aquí describimos un método clínicamente relevante, alta eficiencia y libre de alimentador para reprogramar fibroblastos primarios humanos en células pluripotentes inducidas, usando modificado mRNAs que codifican factores de reprogramación y microRNA maduro-367/302 imita. También se incluyen métodos para evaluar la eficiencia de reprogramación, ampliar las colonias clonales iPSC y confirmar la expresión de los marcadores de pluripotencia TRA-1-60.

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Biología del desarrollo número 141 inducido pluripotent las células de vástago (iPSCs) reprogramación humana modificada mRNAs clínicamente relevantes microRNAs (miRNAs) fibroblastos
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