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Journal / Developmental Biology / RNA-baserade omprogrammering av mänskliga primära …
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
RNA-baserade omprogrammering av mänskliga primära fibroblaster till inducerade pluripotenta stamceller
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RNA-baserade omprogrammering av mänskliga primära fibroblaster till inducerade pluripotenta stamceller

Article DOI: 10.3791/58687-v • 11:39 min • November 26th, 2018
November 26th, 2018 •
1,2,3, 1,2, 1,2,3, 1,2,3
1Department of Dermatology, University of Colorado School of Medicine, Anschutz Medical Campus, 2Charles C. Gates Center for Regenerative Medicine, University of Colorado School of Medicine, Anschutz Medical Campus, 3Stem Cell Biobank and Disease Modeling Core, University of Colorado School of Medicine, Anschutz Medical Campus

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Här beskriver vi en kliniskt relevant, hög verkningsgrad, feeder-fri metod att omprogrammera humana primära fibroblaster till inducerade pluripotenta stamceller använder modifierade mRNA kodning omplanering faktorer och mogen mikroRNA-367/302 härmar. Här ingår också metoder för att bedöma omplanering effektivitet, expandera klonal iPSC kolonier och bekräfta uttryck för pluripotens markören TRA-1-60.

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Fråga 141 mikroRNA (Mirna) inducerad pluripotenta stamceller (iPSCs) omprogrammering mänskliga modifierade mRNA kliniskt relevant utvecklingsbiologi fibroblaster
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