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Journal / Developmental Biology / RNA tabanlı insan birincil fibroblastlar…
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
RNA tabanlı insan birincil fibroblastlar indüklenmiş Pluripotent kök hücre yeniden programlama
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RNA tabanlı insan birincil fibroblastlar indüklenmiş Pluripotent kök hücre yeniden programlama

Article DOI: 10.3791/58687-v • 11:39 min • November 26th, 2018
November 26th, 2018 •
1,2,3, 1,2, 1,2,3, 1,2,3
1Department of Dermatology, University of Colorado School of Medicine, Anschutz Medical Campus, 2Charles C. Gates Center for Regenerative Medicine, University of Colorado School of Medicine, Anschutz Medical Campus, 3Stem Cell Biobank and Disease Modeling Core, University of Colorado School of Medicine, Anschutz Medical Campus

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Burada biz insan birincil fibroblastlar değiştirilmiş mRNA'ların programlama faktörler kodlama kullanarak indüklenmiş pluripotent kök hücre yeniden programlamak için klinik, yüksek verimli, besleyici-Alerjik bir yöntemi açıklamak ve olgun mikroRNA-367/302 taklit eder. Ayrıca programlama verimlilik, değerlendirmek için yöntem klonal IPSC kolonileri genişletin ve pluripotency işaretin TRA-1-60 ifade onaylamak bulunmaktadır.

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Gelişim biyolojisi sayı: 141 Induced pluripotent kök hücreler (iPSCs) yeniden programlama insan değiştirilmiş mRNA'ların klinik mikroRNA (miRNAs) fibroblastlar
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