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Journal / Biochemistry / Enkelt-trins rensning af makromolekylære…
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Biochemistry

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JoVE Journal Biochemistry
Enkelt-trins rensning af makromolekylære komplekser ved hjælp af RNA knyttet til Biotin og et foto-cleavable Linker
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Lipid Index Determination by Liquid Fluorescence Recovery in the Fungal Pathogen Ustilago Maydis
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Enkelt-trins rensning af makromolekylære komplekser ved hjælp af RNA knyttet til Biotin og et foto-cleavable Linker

Article DOI: 10.3791/58697-v • 08:12 min • January 3rd, 2019
January 3rd, 2019 •
1, 1, 1,2
1Integrative Program for Biological and Genome Sciences, University of North Carolina at Chapel Hill, 2Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill

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RNA/protein komplekser renset ved hjælp af botin-streptavidin strategi er elueret løsning på denaturering betingelser i en form, der er uegnet til yderligere rensning og funktionelle analyse. Her, beskriver vi en ændring af denne strategi, der udnytter en foto-cleavable linker i RNA og en blid UV-eluering skridt, fremstilling af indfødte og fuldt funktionel RNA/protein komplekser.

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Biokemi spørgsmålet 143 affinitet rensning RNA/protein komplekser biotin streptavidin foto-cleavable linker UV-eluering U7 snRNP 3' ende behandling
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