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Journal / Biology / 드롭릿 바코드 기반 단일 셀 Transcriptomics 성인 포유류…
JoVE Journal
Biology

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JoVE Journal Biology
드롭릿 바코드 기반 단일 셀 Transcriptomics 성인 포유류 직물의
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드롭릿 바코드 기반 단일 셀 Transcriptomics 성인 포유류 직물의

Article DOI: 10.3791/58709-v • 10:12 min • January 10th, 2019
January 10th, 2019 •
*1,2,3, *2, 2, 2, 1,4, 2, 1,4, 1,2,3
1Hotchkiss Brain Institute, University of Calgary, 2Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary, 3Alberta Children's Hospital Research Institute, University of Calgary, 4Department of Clinical Neurosciences, Cumming School of Medicine, University of Calgary
* These authors contributed equally

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이 프로토콜에서는 일반적인 프로세스를 설명 합니다 및 품질 관리 검사 드롭릿 기반, 높은 처리량 단일 셀 RNA-Seq 준비에 대 한 건강 한 성인 포유류 단일 세포를 준비에 필요한. 시퀀싱 매개 변수, 읽기 정렬 및 다운스트림 단일 셀 bioinformatic 분석 또한 제공 됩니다.

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생물학 문제점 143 단일 셀 RNA-Seq 성인 Transcriptome RNA FACS
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