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Journal / Biology / Droplet streckkodning-baserade enda Cell…
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Biology

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JoVE Journal Biology
Droplet streckkodning-baserade enda Cell transkriptomik av vuxna däggdjur vävnader
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Droplet streckkodning-baserade enda Cell transkriptomik av vuxna däggdjur vävnader

Article DOI: 10.3791/58709-v • 10:12 min • January 10th, 2019
January 10th, 2019 •
*1,2,3, *2, 2, 2, 1,4, 2, 1,4, 1,2,3
1Hotchkiss Brain Institute, University of Calgary, 2Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary, 3Alberta Children's Hospital Research Institute, University of Calgary, 4Department of Clinical Neurosciences, Cumming School of Medicine, University of Calgary
* These authors contributed equally

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Det här protokollet beskriver de allmänna processerna och kvalitetskontroll kontroller som krävs för att förbereda friska vuxna enda däggdjursceller droplet-baserade, hög genomströmning enda cell RNA-Seq preparat. Sekvensering parametrar, finns Läs justering och nedströms encelliga bioinformatiska analyser också.

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Biologi fråga 143 enstaka cell vuxen RNA transkriptom RNA-Seq FACS
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