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Journal / Cancer Research / Undersøgelse af genetiske afhængigheder ved hjælp …
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JoVE Journal Cancer Research
Undersøgelse af genetiske afhængigheder ved hjælp af CRISPR-Cas9-baserede konkurrence Assays
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Undersøgelse af genetiske afhængigheder ved hjælp af CRISPR-Cas9-baserede konkurrence Assays

Article DOI: 10.3791/58710-v • 11:05 min • January 7th, 2019
January 7th, 2019 •
1, 1, 1, 1, 1, 2, 3, 1
1Tumor Initiation and Maintenance Program, Sanford Burnham Prebys Medical Discovery Institute, 2Blood Research Institute, Blood Center of Wisconsin, 3Department of Bioengineering, University of California, San Diego

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Dette manuskript beskriver en grupperet regelmæssigt Interspaced korte palindromiske gentager (CRISPR) CRISPR-Cas9-baseret metode for enkel og hurtig undersøgelse af flere kandidat gener i akut Myeloid leukæmi (AML) celledelingen i rolle parallel. Denne teknik er skalerbar og kan anvendes i andre kræft cellelinjer så godt.

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Kræftforskning spørgsmålet 143 AML CRISPR-Cas9 høj overførselshastighed assay konkurrencedygtig vækst assay DOT1L leukæmi kræft
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