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Immunology and Infection

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JoVE Journal Immunology and Infection
High Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing
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High Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing

Article DOI: 10.3791/58753-v • 07:18 min • January 22nd, 2019
January 22nd, 2019 •
1, 1
1Department of Microbiology and Immunology, University of Melbourne

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A high throughput protocol for functional assessment of HIV efficient reactivation and clearance of latent proviruses is described and applied by testing the impact of interventions on HIV transcription and splicing. Representative results of the effect of latency reversing agents on LTR-driven transcription and splicing are provided.

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High Throughput In Vitro Assessment Latency Reversing Agents HIV Transcription HIV Splicing Protocol LRA HIV MRNA Processing Virus Reactivation Latent Reservoir Cells DMEM Medium FBS Antibiotics 96-well Plate Dual Color Reporter Construct Tat And Rev DNA Serum Free Medium Lipid Reagent Incubation Room Temperature Lipid Reagent DNA Complex Transfection CMV Driven EGFP And DsRed Express DNA Plasmid
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