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JoVE Journal Neuroscience
To-foton billeddannelse af Microglial processer tiltrækning mod ATP eller Serotonin i akut hjernen skiver
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To-foton billeddannelse af Microglial processer tiltrækning mod ATP eller Serotonin i akut hjernen skiver

Article DOI: 10.3791/58788-v • 07:27 min • January 31st, 2019
January 31st, 2019 •
1,2,3, 1,2,3, 1,2,3, 1,2,3, *1,2,3, *1,2,3
1Institut National de la Santé et de la Recherche Médicale (INSERM), UMR-S 1270 Paris, France, 2Sorbonne Université, Paris, France, 3Institut du Fer à Moulin, Paris, France
* These authors contributed equally

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Mikroglia, hjernen, bosiddende immunceller reagere hurtigt med morfologiske ændringer til ændringer i deres miljø. Denne protokol beskriver, hvordan du bruger to-foton mikroskopi til at studere tiltrækning af microglial processer mod serotonin eller ATP i akut hjernen skiver af mus.

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Neurovidenskab spørgsmålet 143 mikroglia multi foton mikroskopi akut hjernen skiver chemoattraction motilitet morfologi serotonin ATP live imaging time-lapse imaging
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