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Journal / Neuroscience / Två-foton avbildning av mikrogliala processerna…
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JoVE Journal Neuroscience
Två-foton avbildning av mikrogliala processerna attraktion mot ATP eller Serotonin i akut hjärnskada skivor
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Två-foton avbildning av mikrogliala processerna attraktion mot ATP eller Serotonin i akut hjärnskada skivor

Article DOI: 10.3791/58788-v • 07:27 min • January 31st, 2019
January 31st, 2019 •
1,2,3, 1,2,3, 1,2,3, 1,2,3, *1,2,3, *1,2,3
1Institut National de la Santé et de la Recherche Médicale (INSERM), UMR-S 1270 Paris, France, 2Sorbonne Université, Paris, France, 3Institut du Fer à Moulin, Paris, France
* These authors contributed equally

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Mikroglia, bosatt immuncellerna i hjärnan, svara snabbt med morfologiska förändringar ändringar av deras miljö. Det här protokollet beskriver hur man använder två-foton mikroskopi för att studera attraktion av mikrogliala processer mot serotonin eller ATP i akut hjärnskada skivor av möss.

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Neurovetenskap fråga 143 mikroglia flera foton mikroskopi akut hjärnskada skivor kemoattraktion motilitet morfologi serotonin ATP levande imaging time-lapse imaging
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