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Journal / Genetics / 从活细胞中的单端粒表达的癌症细胞克隆的生成, 以可视化含有端粒重复的 rna…
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Genetics

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JoVE Journal Genetics
从活细胞中的单端粒表达的癌症细胞克隆的生成, 以可视化含有端粒重复的 rna terra
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从活细胞中的单端粒表达的癌症细胞克隆的生成, 以可视化含有端粒重复的 rna terra

Article DOI: 10.3791/58790-v • 09:13 min • January 17th, 2019
January 17th, 2019 •
1, 1, 1,2, 1
1Laboratory of Cell Biology and Molecular Genetics, Department of Cellular, Computational and Integrative Biology - CIBIO, University of Trento, 2Department of Life Sciences, University of Trieste

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在这里, 我们提出了一个协议, 以生成包含 ms2 序列标记在单个亚端粒的癌细胞克隆。这种方法, 依靠 ms2-gfp 系统, 实现了端粒重复 rna (terra) 的内源性转录记录的可视化表达从一个端粒表达在活细胞中。

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遗传学 第143期 长非编码 rna terra 端粒 癌症 crispric9 ms2-gfp 活细胞成像。
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