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JoVE Journal Neuroscience
Diagnosis of Hirschsprung's Disease by Immunostaining Rectal Suction Biopsies for Calretinin, S100 Protein and Protein Gene Product 9.5
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Diagnosis of Hirschsprung's Disease by Immunostaining Rectal Suction Biopsies for Calretinin, S100 Protein and Protein Gene Product 9.5

Article DOI: 10.3791/58799-v • 05:45 min • April 26th, 2019
April 26th, 2019 •
1, 1, 1, 2, 1
1Department of Pediatric Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 2Division of Gastroenterology, Hepatology and Nutrition, Cincinnati Children's Hospital Medical Center

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Summary
Automatic Translation

This protocol describes the process of immunostaining rectal suction biopsies for calretinin, S100 protein, and protein gene product 9.5. This novel adjuvant diagnostic method for Hirschsprung's disease has preferable sensitivity and specificity rates.

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Hirschsprung's Disease Immunostaining Rectal Suction Biopsies Calretinin S100 Protein Protein Gene Product 9.5 Diagnosis Method High Sensitivity Specificity Rates Protocol Mijing Fang Technician Laboratory Fixing Paraformaldehyde Dehydration Pathological Tissue Dehydrator Paraffin Blocks Microtome Section Plane Flat Plan Glass Slides Oven Baking Deparaffinized Slides Xylene Changes Ethanol Immersions Reverse Osmosis Purified Water Antigen Retrieval
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