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Journal / Biochemistry / Rekombinant füzyon protein flüoresan proteaz…
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Rekombinant füzyon protein flüoresan proteaz tahlil Platform ve onların jel Renaturation kullanımı
Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase
Journal (Biochemistry)
Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase
Identifying the Binding Proteins of Small Ligands with the Differential Radial Capillary Action of Ligand Assay (DRaCALA)
Journal (Biochemistry)
Identifying the Binding Proteins of Small Ligands with the Differential Radial Capillary Action of Ligand Assay (DRaCALA)
 
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Rekombinant füzyon protein flüoresan proteaz tahlil Platform ve onların jel Renaturation kullanımı

Article DOI: 10.3791/58824-v • 19:23 min • January 16th, 2019
January 16th, 2019 •
*1,2, *1, 1, 1, 1
1Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, 2Biotechnology Research Department, Gedeon Richter Plc
* These authors contributed equally

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Burada, N-terminal hexahistidine/maltoz-bağlayıcı protein ve nikel-nitrilotriacetic yüzeye bağlı floresan protein erimiş rekombinant yüzeylerde kullanan bir son zamanlarda geliştirilen proteaz tahlil platform ayrıntılı yordam mevcut asit manyetik özel boncuk. Bir sonraki jel analiz Sodyum Lauryl Sülfat-polyacrylamide jel elektroforez tarafından ayrılmış tahlil örnekleri de sunulmaktadır.

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Biyokimya sayı 143 rekombinant füzyon protein substrat proteaz tahlil Ni-NTA manyetik özel boncuk floresan protein jel renaturation insan immün yetmezlik virüsü tip 1 proteaz
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