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Immunology and Infection

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JoVE Journal Immunology and Infection
Fastsettelse av regulatoriske T-celle delsett i Murine Thymus, bukspyttkjertelen drenering lymfeknute og milt bruker flowcytometri
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Fastsettelse av regulatoriske T-celle delsett i Murine Thymus, bukspyttkjertelen drenering lymfeknute og milt bruker flowcytometri

Article DOI: 10.3791/58848-v • 08:06 min • February 27th, 2019
February 27th, 2019 •
1, 1, 1, 1
1Department of Medical Cell Biology, Uppsala University

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Her presenterer vi en protokoll for å forberede enkeltceller fra murine thymus, bukspyttkjertelen drenering lymfeknute og milt å ytterligere studere disse celler ved hjelp av flowcytometri. I tillegg ble denne protokollen brukt for å bestemme delsett av regulatoriske T celler ved hjelp av flowcytometri.

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Immunologi og infeksjon problemet 144 enkeltcelle forberedelse regulatoriske T-celler flowcytometri Foxp3 Helios Neuropilin 1
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