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Journal / Neuroscience / Sondering nicotinsyre acetylcholin Receptor…
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Neuroscience

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JoVE Journal Neuroscience
Sondering nicotinsyre acetylcholin Receptor funktion i mus hjernen skiver via Laser Flash fotolyse Photoactivatable nikotin
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Sondering nicotinsyre acetylcholin Receptor funktion i mus hjernen skiver via Laser Flash fotolyse Photoactivatable nikotin

Article DOI: 10.3791/58873-v • 10:48 min • January 25th, 2019
January 25th, 2019 •
1, 2, 3, 3, 1
1Department of Pharmacology, Northwestern University Feinberg School of Medicine, 2Department of Physiology, Northwestern University Feinberg School of Medicine, 3Janelia Research Campus, Howard Hughes Medical Institute

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Denne artikel præsenterer en metode til at studere nicotinsyre acetylcholin receptorer (nAChRs) i mus hjernen skiver af nikotin uncaging. Når kombineret med samtidige patch klemme optagelse og 2-foton laser scanning mikroskopi, forbinder nikotin uncaging nicotinsyre receptoren funktion med cellulære morfologi, giver en dybere forståelse af kolinerge neurobiologi.

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Neurovidenskab spørgsmålet 143 fotolyse uncaging nikotin kolinerge nicotinsyre 2-foton Elektrofysiologi imaging receptor acetylcholin
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