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Journal / Neuroscience / Verifiserer nikotinsyre acetylcholin…
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Neuroscience

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JoVE Journal Neuroscience
Verifiserer nikotinsyre acetylcholin reseptor-funksjonen i musen hjernen skiver via Laser Flash Photolysis Photoactivatable nikotin
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Verifiserer nikotinsyre acetylcholin reseptor-funksjonen i musen hjernen skiver via Laser Flash Photolysis Photoactivatable nikotin

Article DOI: 10.3791/58873-v • 10:48 min • January 25th, 2019
January 25th, 2019 •
1, 2, 3, 3, 1
1Department of Pharmacology, Northwestern University Feinberg School of Medicine, 2Department of Physiology, Northwestern University Feinberg School of Medicine, 3Janelia Research Campus, Howard Hughes Medical Institute

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Denne artikkelen presenterer en metode for å studere nikotinsyre acetylkolin reseptorer (nAChRs) i musen hjernen skiver av nikotin uncaging. Når kombinert med samtidige oppdateringen klemme opptak og 2-fotonet laserskanning mikroskopi, forbinder nikotin uncaging nikotinsyre reseptoren funksjonen med cellular morfologi, gir en dypere forståelse av cholinergic nevrobiologi.

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Nevrovitenskap problemet 143 Photolysis uncaging nikotin cholinergic nikotinsyre 2-fotonet elektrofysiologi bildebehandling reseptor acetylcholine
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