JoVE Journal
Chemistry
Chemistry
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大腸菌の無細胞タンパク質合成のベース
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Article
DOI:
10.3791/58882-v
•
09:45 min
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February 25th, 2019
大腸菌の無細胞タンパク質合成のベース: 堅牢な柔軟性とアクセス可能なプラットフォーム技術のためのプロトコル
February 25th, 2019
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Max Z. Levine*1,2, Nicole E. Gregorio*2,3, Michael C. Jewett4,5,6,7, Katharine R. Watts2,3, Javin P. Oza2,3
1Department of Biological Sciences, California Polytechnic State University, San Luis Obispo, 2Center for Applications in Biotechnology, California Polytechnic State University, San Luis Obispo, 3Department of Chemistry and Biochemistry, California Polytechnic State University, 4Department of Chemical and Biological Engineering, Northwestern University, 5Chemistry of Life Processes Institute, Northwestern University, 6Center for Synthetic Biology, Northwestern University, 7Interdisciplinary Biological Sciences Program, Northwestern University
* These authors contributed equally
Chapters
Summary
Please note that all translations are automatically generated.
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このプロトコルの詳細手順、コスト、および大腸菌を生成に必要な装置・細胞抽出液をベースし、4 日間以内の in vitroタンパク質合成反応を実装します。広範なアプリケーションは、このプラットフォームの柔軟な性質を活用するには、と最適化することができます反応条件について検討します。