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Escherichia coli-base libres de células síntesis de la proteína: protocolos para una tecnología de plataforma robusta, flexible y accesible

Article DOI: 10.3791/58882-v • 09:45 min • February 25th, 2019
February 25th, 2019 •
*1,2, *2,3, 4,5,6,7, 2,3, 2,3
1Department of Biological Sciences, California Polytechnic State University, San Luis Obispo, 2Center for Applications in Biotechnology, California Polytechnic State University, San Luis Obispo, 3Department of Chemistry and Biochemistry, California Polytechnic State University, 4Department of Chemical and Biological Engineering, Northwestern University, 5Chemistry of Life Processes Institute, Northwestern University, 6Center for Synthetic Biology, Northwestern University, 7Interdisciplinary Biological Sciences Program, Northwestern University
* These authors contributed equally

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Este protocolo detalla los pasos, costos y equipamiento necesario para generar e. coli-basado en extractos celulares e implementar en vitro reacciones de la síntesis de proteína dentro de 4 días o menos. Para aprovechar la naturaleza flexible de esta plataforma para aplicaciones generales, se discuten las condiciones de reacción que pueden ser adaptadas y optimizadas.

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Química número 144 sin células proteína síntesis PFC en vitro expresión proteica alto rendimiento proteína síntesis TX-TL biología sintética libre de célula metabólica ingeniería CFME
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