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Analisi di efflusso di High-throughput Nitrobenzoxadiazole-labeled colesterolo
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Analisi di efflusso di High-throughput Nitrobenzoxadiazole-labeled colesterolo

Article DOI: 10.3791/58891-v • 08:18 min • January 7th, 2019
January 7th, 2019 •
1, 1, 1
1Group of Genomics and Pharmacogenomics in HIV, Laboratory of Retrovirology and Viral Immunopathogenesis, IDIBAPS, Hospital Clínic de Barcelona

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Misurazione della capacità di efflusso di colesterolo in vitro di siero o plasma in modelli cellulari del macrofago è uno strumento promettente come biomarcatore per aterosclerosi. Nello studio presente, abbiamo ottimizzare e standardizzare un metodo di efflusso di colesterolo-NBD fluorescente e sviluppare un'analisi di alto-rendimento utilizzando piastre da 96 pozzetti.

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Medicina numero 143 NBD-colesterolo macrofago HDL efflusso di colesterolo fluorescenza apolipoproteina B-vuotati del siero aterosclerosi trasporto inverso del colesterolo
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