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Isolatie, zuivering en differentiatie van osteoclast precursoren van het Rat beenmerg
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Isolatie, zuivering en differentiatie van osteoclast precursoren van het Rat beenmerg

Article DOI: 10.3791/58895-v • 11:11 min • May 19th, 2019
May 19th, 2019 •
1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 3, 3, 1,2,4
1Laboratory of New Techniques of Restoration & Reconstruction, Institute of Traumatology & Orthopedics, Nanjing University of Chinese Medicine, 2TCM Nursing Intervention Laboratory of Chronic Disease Key Laboratory, Nanjing University of Chinese Medicine, 3Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, 4Department of Traumatology and Orthopedics, Affiliated Hospital of Nanjing University of Chinese Medicine

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Osteoclasten zijn weefsel-specifieke macrofagen polykaryons afgeleid van de monocyten-macrofagen afstamming van hematopoietische stamcellen. In dit protocol wordt beschreven hoe u beenmergcellen isoleren zodat grote hoeveelheden osteoclasten worden verkregen terwijl het risico op ongevallen in traditionele methoden wordt verminderd.

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Biologie osteoclast osteoporose receptor Activator voor nucleaire factor-κB ligand (RANK) Bone remodeling celbiologie isolatie Bone resorptie
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