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Påvisning av Protease aktivitet av fluorescerende peptid Zymography
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Påvisning av Protease aktivitet av fluorescerende peptid Zymography

Article DOI: 10.3791/58938-v • 09:56 min • January 20th, 2019
January 20th, 2019 •
1,2, 1, 1,2
1Comprehensive Cancer Center, James Cancer Hospital and Solove Research Center, Ohio State University, 2Department of Biomedical Engineering, College of Engineering, Ohio State University

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Her presenterer vi en detaljert protokoll for en modifisert zymographic teknikk som brukes fluorescerende peptider som nedbrytbart underlaget i stedet for native proteiner. Geleelektroforese av biologiske prøver i fluorescerende peptid zymograms gjør påvisning av et bredere spekter av proteaser enn forrige zymographic teknikker.

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Kreftforskning problemet 143 Zymography fluorescerende peptid Protease matrise Metalloproteinase (MMP) Kovalente Crosslinking kreft betinget Media geleelektroforese
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