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Journal / Neuroscience / Identificación de marcadores de superficie…
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Neuroscience

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JoVE Journal Neuroscience
Identificación de marcadores de superficie celular de células madre neuronales primarias y células progenitoras por etiquetado metabólico de sialoglicano
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Identificación de marcadores de superficie celular de células madre neuronales primarias y células progenitoras por etiquetado metabólico de sialoglicano

Article DOI: 10.3791/58945-v • 11:39 min • September 7th, 2019
September 7th, 2019 •
*1, *2, 1
1Brain and Spinal Cord Innovative Research Center of Tongji Hospital, School of Life Sciences and Technology, Tongji University and Frontier Science Research Center for Stem Cells of Ministry of Education, 2College of Chemistry and Molecular Engineering, Peking-Tsinghua Center for Life Sciences, Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, and Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University
* These authors contributed equally

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Aquí se presenta un protocolo que combina un sistema de cocultivo endotelial neuronal in vitro e incorporación metabólica de sialoglicano con grupos funcionales bioortogonales para expandir las células primarias del tallo neural y del progenitor y etiquetar su superficie sialoglycoproteínas para el análisis de imágenes o espectrometría de masas de marcadores de superficie celular.

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Neurociencia Número 151 Etiquetado de glicano metabólico ácidos siálicos reacción bioortogonal células madre neurales células progenitoras marcadores de superficie células endoteliales proteome etiquetado químico corteza cerebral
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