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Neuroscience

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JoVE Journal Neuroscience
Identificando marcadores de superfície celular da haste neural primária e células progenitoras por rotulagem metabólica de Sialoglycan
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Identificando marcadores de superfície celular da haste neural primária e células progenitoras por rotulagem metabólica de Sialoglycan

Article DOI: 10.3791/58945-v • 11:39 min • September 7th, 2019
September 7th, 2019 •
*1, *2, 1
1Brain and Spinal Cord Innovative Research Center of Tongji Hospital, School of Life Sciences and Technology, Tongji University and Frontier Science Research Center for Stem Cells of Ministry of Education, 2College of Chemistry and Molecular Engineering, Peking-Tsinghua Center for Life Sciences, Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, and Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University
* These authors contributed equally

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Aqui apresentamos um protocolo que combina um sistema de cocultura neural-endotelial in vitro e incorporação metabólica de sialoglicano com grupos funcionais bioortogonais para expandir as células primárias de tronco neural e progenitoras e rotular sua superfície sialoglycoproteínas para a análise da imagem latente ou da massa-espectrometria de marcadores da superfície da pilha.

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Neurociência edição 151 rotulagem do glicana metabólico ácidos siálico reação do pilhas de haste neural pilhas do progenitor marcadores de superfície pilhas endothelial Proteome rotulagem química córtice cerebral
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