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Journal / Neuroscience / Identifikation af celleoverflade markører for…
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JoVE Journal Neuroscience
Identifikation af celleoverflade markører for primær neurale stamme og stamceller ved metabolisk mærkning af Sialoglycan
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Identifikation af celleoverflade markører for primær neurale stamme og stamceller ved metabolisk mærkning af Sialoglycan

Article DOI: 10.3791/58945-v • 11:39 min • September 7th, 2019
September 7th, 2019 •
*1, *2, 1
1Brain and Spinal Cord Innovative Research Center of Tongji Hospital, School of Life Sciences and Technology, Tongji University and Frontier Science Research Center for Stem Cells of Ministry of Education, 2College of Chemistry and Molecular Engineering, Peking-Tsinghua Center for Life Sciences, Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, and Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University
* These authors contributed equally

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Præsenteret her er en protokol, der kombinerer en in vitro neurale-Endothelial Co-kultur system og metabolisk inkorporering af sialoglycan med bioorthogonal funktionelle grupper til at udvide primære neurale stamme og stamcelle celler og mærke deres overflade sialoglycoproteiner til billeddannelse eller massespektrometri analyse af celleoverflade markører.

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Neurovidenskab metabolisk Les mærkning sialsyrer bioorthogonal reaktion neurale stamceller progenitorceller overflade markører endotelceller proteome kemisk mærkning hjernebarken
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