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Journal / Immunology and Infection / Identifiering av mediatorer av T-cells Receptor…
JoVE Journal
Immunology and Infection

This content is Open Access.

JoVE Journal Immunology and Infection
Identifiering av mediatorer av T-cells Receptor signalering via Screening av kemiska hämmare bibliotek
Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins
Journal (Immunology and Infection)
Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins
Activation and Conjugation of Soluble Polysaccharides using 1-Cyano-4-Dimethylaminopyridine Tetrafluoroborate (CDAP)
Journal (Immunology and Infection)
Activation and Conjugation of Soluble Polysaccharides using 1-Cyano-4-Dimethylaminopyridine Tetrafluoroborate (CDAP)
 
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Identifiering av mediatorer av T-cells Receptor signalering via Screening av kemiska hämmare bibliotek

Article DOI: 10.3791/58946-v • 08:49 min • January 22nd, 2019
January 22nd, 2019 •
*1, *2,3, *1, 1, 1,4
1Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 2Singapore Immunology Network, A*STAR, 3Curiox Biosystems, 4Department of Immunobiology, Rega Institute for Medical Research, Katholieke Universiteit (KU) Leuven
* These authors contributed equally

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Detta dokument använder en flow-flödescytometri-baserad analys till skärmen bibliotek av kemiska hämmare för identifiering av hämmare och sina mål som påverkar T-cells receptor signalering. Metoderna som beskrivs här kan också utökas för hög genomströmning filmvisningar.

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Immunologi och infektion fråga 143 kaspas-3 aktivering kinashämmare bibliotek screening TCR signalering T-cells aktivering thymocyte urval
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