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JoVE Journal
Immunology and Infection

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双氟铬流式细胞仪对七种免疫细胞子集的判别
 
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双氟铬流式细胞仪对七种免疫细胞子集的判别

Article DOI: 10.3791/58955-v 10:58 min March 5th, 2019
March 5th, 2019

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Summary

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在这里, 我们提出了一个流式细胞仪协议, 以识别 cd4+和 cd8 + t 细胞, γ t细胞, b 细胞, nk 细胞和单核细胞在人外周血中只使用两个荧光色素, 而不是七个。通过这种方法, 可以在大多数流式细胞仪上记录五个附加标记。

Tags

免疫学与感染 第145期 流式细胞术 免疫学 免疫表型 人外周血 荧光色素 患者 多参数分析。
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