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Journal / Immunology and Infection / İki-fluorochrome tarafından yedi bağışıklık hücre …
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Immunology and Infection

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JoVE Journal Immunology and Infection
İki-fluorochrome tarafından yedi bağışıklık hücre alt kümeleri ayrımcılık Akış Sitometresi
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Culture Methods to Determine the Limit of Detection and Survival in Transport Media of Campylobacter Jejuni in Human Fecal Specimens
Journal (Immunology and Infection)
Culture Methods to Determine the Limit of Detection and Survival in Transport Media of Campylobacter Jejuni in Human Fecal Specimens
 
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İki-fluorochrome tarafından yedi bağışıklık hücre alt kümeleri ayrımcılık Akış Sitometresi

Article DOI: 10.3791/58955-v • 10:58 min • March 5th, 2019
March 5th, 2019 •
1, 2
1Oncology Research, Medimmune, LLC, 2Division of Rheumatology, Johns Hopkins University School of Medicine

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Burada, CD4 tanımlamak için bir akış sitometrik protokol mevcut+ ve CD8+ T hücreleri, γδ T hücreleri, B hücreler, NK hücreleri ve monosit yedi yerine sadece iki fluorochromes kullanarak insan periferik kan içinde. Bu yaklaşım ile beş ek işaretleri çoğu akışı cytometers üzerinde kaydedilir.

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İmmünoloji ve enfeksiyon sayı: 145 Akış Sitometresi İmmünoloji immunophenotyping insan periferik kan fluorochrome sabırlı multiparametric analizi.
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