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Neuroscience

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JoVE Journal Neuroscience
Culture de cellules primaires de neurones GABAergiques ou glutamatergiques purifiés établis par tri cellulaire activé par fluorescence
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Culture de cellules primaires de neurones GABAergiques ou glutamatergiques purifiés établis par tri cellulaire activé par fluorescence

Article DOI: 10.3791/58974-v • 10:57 min • June 6th, 2019
June 6th, 2019 •
1, 1, 2, 3, 1
1Institut für Integrative Neuroanatomie, Charité, Universitätsmedizin Berlin, 2Department for Cytometry and Cellsorting, German Rheumatism Research Center, Leibniz Institute, 3Departments of Genetic and Behavioral Neuroscience, Graduate School of Medicine, Gunma University

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Ce protocole décrit une méthode basée sur le tri cellulaire pour la purification et la culture des neurones GABAergiques fluorescents ou glutamatergiques du néocortex et de l’hippocampe de souris ou de rats postnatals.

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Neurosciences numéro 148 FACS culture cellulaire neurones GABAergiques interneurones neurones glutamatergiques cellules principales neurones primaires Hippocampus cortex souris rat
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