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Neuroscience

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JoVE Journal Neuroscience
Primär cell kultur av renade GABAergic eller Glutamatergic nerv celler etablerad genom Fluorescence-aktive rad cell sortering
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Primär cell kultur av renade GABAergic eller Glutamatergic nerv celler etablerad genom Fluorescence-aktive rad cell sortering

Article DOI: 10.3791/58974-v • 10:57 min • June 6th, 2019
June 6th, 2019 •
1, 1, 2, 3, 1
1Institut für Integrative Neuroanatomie, Charité, Universitätsmedizin Berlin, 2Department for Cytometry and Cellsorting, German Rheumatism Research Center, Leibniz Institute, 3Departments of Genetic and Behavioral Neuroscience, Graduate School of Medicine, Gunma University

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Detta protokoll beskriver en cell sortering baserad metod för rening och kultur av fluorescerande GABAergic eller glutamaterga nerv celler från neocortex och Hippocampus av postnatal möss eller råttor.

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Neurovetenskap FACS cell kultur GABAergic nerv celler interneuroner glutamaterga nerv celler huvudsakliga celler primära nerv celler Hippocampus cortex mus råtta
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