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Journal / Genetics / Ingegneria genetica di Dictyostelium discoideum…
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Genetics

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JoVE Journal Genetics
Ingegneria genetica di Dictyostelium discoideum cellule basata sulla selezione e crescita sui batteri
Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation
Journal (Genetics)
Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation
Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
Journal (Genetics)
Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
 
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Ingegneria genetica di Dictyostelium discoideum cellule basata sulla selezione e crescita sui batteri

Article DOI: 10.3791/58981-v • 06:08 min • January 25th, 2019
January 25th, 2019 •
1, 2, 1, 3, 3, 4,5, 1, 1
1MRC Laboratory of Molecular Biology, 2Department of Molecular and Cell Biology, University of Connecticut, 3Cancer Research UK Beatson Institute Glasgow, 4MRC Laboratory for Molecular Cell Biology, University College London, 5Department of Cell and Developmental Biology, University College London

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Dictyostelium discoideum è un organismo modello popolare per studiare i complessi processi cellulari come la migrazione cellulare, endocitosi e sviluppo. L'utilità dell'organismo è dipenda sulla fattibilità di manipolazione genetica. Qui, presentiamo metodi per transfect Dictyostelium discoideum cellule che superare i limiti esistenti delle cellule di coltura in terreno liquido.

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Genetica problema 143 Dictyostelium trasfezione knock-out knock-in plasmidi extracromosomico act5 sovraespressione chemiotassi motilità Macropinocitosi
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