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JoVE Journal
Immunology and Infection

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Produktion af Pseudotyped partikler at studere højpatogen coronavirus i biosikkerhed niveau 2 omgivelser
 
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Produktion af Pseudotyped partikler at studere højpatogen coronavirus i biosikkerhed niveau 2 omgivelser

Article DOI: 10.3791/59010-v 08:40 min March 1st, 2019
March 1st, 2019

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Summary

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Click here for the English version.

Vi præsenterer her, en protokol til at generere pseudotyped partikler i BSL-2 omgivelser indarbejde spike protein af højpatogene virus Mellemøsten respiratorisk syndrom og svær Akut respiratorisk syndrom coronavirus. Disse pseudotyped partikler indeholder et luciferase reporter gen giver mulighed for kvantificering af virus ibrugtagning vært målceller.

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Kræftforskning sag 145 Pseudotyped partikel pseudovirion coronavirus CoV spike protein alvorlig akut luftvejssyndrom coronavirus SARS-CoV Mellemøsten luftvejssyndrom coronavirus MERS-CoV murine leukæmi virus MLV
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