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Journal / Immunology and Infection / Produktion von Pseudotyped Partikel hoch…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Produktion von Pseudotyped Partikel hoch pathogene Coronaviren in eine Einstellung "Biosafety Level 2" zu studieren
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Produktion von Pseudotyped Partikel hoch pathogene Coronaviren in eine Einstellung "Biosafety Level 2" zu studieren

Article DOI: 10.3791/59010-v • 08:40 min • March 1st, 2019
March 1st, 2019 •
1,2, 3, 3, 4, 3,5, 3, 1
1Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, 2INRA, Virologie et Immunologie Moléculaires, 3Robert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University, 4Department of Microbiology, College of Agricultural and Life Sciences, Cornell University, 5Horae Gene Therapy Center, University of Massachusetts Medical School

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Hier präsentieren wir Ihnen ein Protokoll zur pseudotyped Partikel in eine Einstellung "BSL-2" unter Einbeziehung der Spike-Protein des hoch pathogenen Viren Nahen Osten respiratorisches Syndrom und Schweres Akutes Respiratorisches Syndrom Coronaviren zu generieren. Diese pseudotyped Partikel enthalten eine Luciferase Reporter-gen ermöglicht die Quantifizierung der Virus Eintritt in Zielzellen Host.

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Frage 145 Pseudotyped Partikel Pseudovirion Cancer Research Coronavirus CoV spike Protein Schweres Akutes Respiratorisches Syndrom Corona-Virus SARS-CoV Nahen Osten respiratorisches Syndrom Coronavirus MERS-CoV murine Leukämie Virus MLV
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