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JoVE Journal Genetics
Mit In-vitro- und In der Zelle Form zu untersuchen, kleines Molekül induzierte Pre-mRNA Strukturveränderungen
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Mit In-vitro- und In der Zelle Form zu untersuchen, kleines Molekül induzierte Pre-mRNA Strukturveränderungen

Article DOI: 10.3791/59021-v • 11:58 min • January 30th, 2019
January 30th, 2019 •
1, 2, 1
1Calibr, The Scripps Research Institute, 2Department of Integrative Structural and Computational Biology, The Scripps Research Institute

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Detaillierte Protokolle für beide in vitro und in-Cell selektive 2'-Hydroxyl-Acylation von Primer Extension (Form) Experimente zur Bestimmung der sekundären Struktur der Pre-mRNA-Sequenzen von Interesse im Beisein einer RNA-targeting niedermolekularer analysiert werden in diesem Artikel vorgestellt.

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