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Journal / Biochemistry / Fractionnement de cellules des cellules U937 en…
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Biochemistry

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JoVE Journal Biochemistry
Fractionnement de cellules des cellules U937 en l’Absence de Centrifugation à haute vitesse
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Fractionnement de cellules des cellules U937 en l’Absence de Centrifugation à haute vitesse

Article DOI: 10.3791/59022-v • 06:53 min • January 18th, 2019
January 18th, 2019 •
1, 1, 1, 1, 1, 1
1Department of Basic and Clinical Sciences, Albany College of Pharmacy and Health Sciences

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Nous présentons ici un protocole visant à isoler les membrane plasmique, cytoplasme et les mitochondries des cellules U937 sans l’utilisation de la centrifugation à grande vitesse. Cette technique peut servir à purifier les fractions subcellulaires pour un examen subséquent de la localisation de la protéine par immunoblotting.

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Biochimie numéro 143 fractionnement cellulaire Membrane cytoplasme organites Centrifugation mitochondries U937
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